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h3k9me3 w45a biosensor  (Addgene inc)


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    Addgene inc h3k9me3 w45a biosensor
    Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 <t>(W45A)</t> biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and <t>H3K9me3</t> biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.
    H3k9me3 W45a Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h3k9me3 w45a biosensor/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    h3k9me3 w45a biosensor - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding"

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding

    Journal: iScience

    doi: 10.1016/j.isci.2025.113743

    Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.
    Figure Legend Snippet: Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.

    Techniques Used: Mutagenesis



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    Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 <t>(W45A)</t> biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and <t>H3K9me3</t> biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.
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    (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n= 20 cells) in response to stimulation with 100 ng/ml EGF at 6 min. (C) The response in (B) normalized to the average values of the first 3 time points. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and <t>H3K9me3</t> (mean ± SEM, n= 20 cells) in response to 5 μM TCP over 48 hours. (F) The response in (E) normalized to the average values of the first 3 time points.
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    Image Search Results


    Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.

    Journal: iScience

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding

    doi: 10.1016/j.isci.2025.113743

    Figure Lengend Snippet: Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.

    Article Snippet: H3K9me3 (W45A) biosensor , Addgene , Cat#120808.

    Techniques: Mutagenesis

    Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.

    Journal: iScience

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding

    doi: 10.1016/j.isci.2025.113743

    Figure Lengend Snippet: Construction of FRET calibration standards (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to 100 ng/mL EGF added at 6 min. (C) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n = 20 cells) in response to FAK inhibitor VS-6063 over 24 h. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 (W45A) biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 biosensor (mean ± SEM, n = 20 cells) in response to 5 μM TCP over 48 h.

    Article Snippet: H3K9me3 biosensor , Addgene , Cat#120802.

    Techniques: Mutagenesis

    (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n= 20 cells) in response to stimulation with 100 ng/ml EGF at 6 min. (C) The response in (B) normalized to the average values of the first 3 time points. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 (mean ± SEM, n= 20 cells) in response to 5 μM TCP over 48 hours. (F) The response in (E) normalized to the average values of the first 3 time points.

    Journal: bioRxiv

    Article Title: Calibration of FRET-based biosensors using multiplexed biosensor barcoding

    doi: 10.1101/2024.09.04.610346

    Figure Lengend Snippet: (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n= 20 cells) in response to stimulation with 100 ng/ml EGF at 6 min. (C) The response in (B) normalized to the average values of the first 3 time points. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 (mean ± SEM, n= 20 cells) in response to 5 μM TCP over 48 hours. (F) The response in (E) normalized to the average values of the first 3 time points.

    Article Snippet: Plasmids for transient expression were obtained from Addgene: EKAR (#18679), CytoFAK (#78300), H3K9me3 biosensor (#120802), and H3K9me3 (W45A) biosensor (#120808).

    Techniques: Mutagenesis

    (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n= 20 cells) in response to stimulation with 100 ng/ml EGF at 6 min. (C) The response in (B) normalized to the average values of the first 3 time points. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 (mean ± SEM, n= 20 cells) in response to 5 μM TCP over 48 hours. (F) The response in (E) normalized to the average values of the first 3 time points.

    Journal: bioRxiv

    Article Title: Calibration of FRET-based biosensors using multiplexed biosensor barcoding

    doi: 10.1101/2024.09.04.610346

    Figure Lengend Snippet: (A) Construction of FRET-ON calibration standard by Y349F mutation of Cyto-FAK. (B) Plot of YFP/CFP ratio of CytoFAK and FRET-ON (mean ± SEM, n= 20 cells) in response to stimulation with 100 ng/ml EGF at 6 min. (C) The response in (B) normalized to the average values of the first 3 time points. (D) Construction of FRET-OFF calibration standard by removal of the H3 domain of the H3K9me9 biosensor. (E) Plot of YFP/CFP ratio of FRET-OFF and H3K9me3 (mean ± SEM, n= 20 cells) in response to 5 μM TCP over 48 hours. (F) The response in (E) normalized to the average values of the first 3 time points.

    Article Snippet: Plasmids for transient expression were obtained from Addgene: EKAR (#18679), CytoFAK (#78300), H3K9me3 biosensor (#120802), and H3K9me3 (W45A) biosensor (#120808).

    Techniques: Mutagenesis